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Product Includes Quantity (with Count) Solution Color
Phospho-Tyrosine Mouse mAb (P-Tyr-100) 9411 30 µl
HTScan® Tyrosine Kinase Buffer (4X) 9805 15 ml
DTT (1000X) 80 µl
ATP (10 mM) 9804 1 ml

Product Description

The kit provides a means of performing kinase activity assays with recombinant human IGF-IR kinase. It includes active IGF-IR kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine antibody for detection of the phosphorylated form of the substrate peptide.


Molecular Formula:

Peptide substrate, Biotin-peptide: 2,005 Daltons. GST-IGF-IR Kinase: 77 kDa.


Peptide Core Sequence:

GEY*VN


Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).


1.  Adams, T.E. et al. (2000) Cell. Mol. Life Sci. 57, 1050-1093.

2.  Baserga, R. et al. (2000) Oncogene 19, 5574-5581.

3.  Scheidegger, K.J. et al. (2000) J. Biol. Chem. 275, 38921-38928.

4.  Hernandez-Sanchez, C. et al. (1995) J. Biol. Chem. 270, 29176-29181.

5.  Lopaczynski, W. et al. (2000) Biochem. Biophys. Res. Commun. 279, 955-960.

6.  Baserga, R. et al. (1999) Exp. Cell Res. 253, 1-6.

7.  White, M.F. et al. (1985) J. Biol. Chem. 260, 9470-9478.

8.  White, M.F. et al. (1988) J. Biol. Chem. 263, 2969-2980.


Entrez-Gene Id 3480
Swiss-Prot Acc. P08069

Data Sheets & Documentation


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DELFIA® is a registered trademark of PerkinElmer, Inc.