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Product Includes Quantity (with Count) Solution Color
DTT (1000X) 80 µl
ATP (10 mM) 9804 1 ml

Product Description

The kit provides a means of performing kinase activity assays with recombinant murine Jak2 kinase. It includes active Jak2 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine antibody for detection of the phosphorylated form of the substrate peptide.

Molecular Formula:

FLT3 (Tyr589): 1,945 Daltons, GST-Jak2 Kinase: 71 kDa Daltons.

Peptide Core Sequence:


Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

1.  Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.

2.  VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.

3.  Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.

4.  Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.

5.  Darnell, J.E. (1997) Science 277, 1630-5.

Entrez-Gene Id 3717
Swiss-Prot Acc. O60674

Data Sheets & Documentation

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DELFIA is a registered trademark of PerkinElmer, Inc.

HTScan® Jak2 Kinase Assay Kit