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Product Includes Quantity (with Count) Solution Color
DTT (1000X) 80 µl
ATP (10 mM) 9804 1 ml

Product Description

The kit provides a means of performing kinase activity assays with recombinant human c-Kit kinase. It includes active c-Kit kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine antibody for detection of the phosphorylated form of the substrate peptide.

Molecular Formula:

Peptide substrate, Biotin-KDR (Tyr996): 2,164 Daltons, GST-c-Kit Kinase: 75 KDa.

Peptide Core Sequence:


c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).

1.  Martin, F.H. et al. (1990) Cell 63, 203-11.

2.  Yarden, Y. et al. (1987) EMBO J 6, 3341-51.

3.  Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.

4.  Hirota, S. et al. (1998) Science 279, 577-80.

5.  Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.

6.  Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.

7.  Nocka, K. et al. (1990) EMBO J 9, 1805-13.

Entrez-Gene Id 3815
Swiss-Prot Acc. P10721

Data Sheets & Documentation

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DELFIA is a registered trademark of PerkinElmer, Inc.

HTScan® c-Kit Kinase Assay Kit