Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity (with Count) Solution Color
Phospho-Tyrosine Mouse mAb (P-Tyr-100) 9411 30 µl
HTScan® Tyrosine Kinase Buffer (4X) 9805 15 ml
DTT (1000X) 80 µl
ATP (10 mM) 9804 1 ml
Gastrin Precursor (Tyr87) Biotinylated Peptide 1310 1.25 ml

Product Description

The kit provides a means of performing kinase activity assays with recombinant human VEGFR-2 kinase. It includes active VEGFR-2 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine antibody for detection of the phosphorylated form of the substrate peptide.


Molecular Formula:

Peptide substrate, Biotin-Gastrin Precursor (Tyr87) peptide: 2,853Daltons. GST-VEGFR-2 Kinase: 110 kDa.


Peptide Core Sequence:

EAY*GW


Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).


1.  Meyer, M. et al. (1999) EMBO J 18, 363-74.

2.  Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.

3.  Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.

4.  Takahashi, T. et al. (2001) EMBO J 20, 2768-78.

5.  Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.

6.  Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.

7.  Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.

8.  Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.


Entrez-Gene Id 3791
Swiss-Prot Acc. P35968

Data Sheets & Documentation


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DELFIA® is a registered trademark of PerkinElmer, Inc.