Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Description

The kit provides a means of performing kinase activity assays with recombinant human Abl1 kinase. It includes active Abl1 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine monoclonal antibody for detection of the phosphorylated form of the substrate peptide.


Molecular Formula:

Peptide substrate, Biotin-Signal Transduction Protein (Tyr160): 1830 Daltons. GST-Abl1 Kinase: 76 kDa.


Peptide Core Sequence:

GIY*DV


The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion, and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation; PDGF stimulation; and binding to c-Jun, Nck, and RFX1 (2,4). The in vivo mechanism for regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation at Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation at Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6).


1.  Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.

2.  Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182.

3.  Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.

4.  Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16.

5.  Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.

6.  Pluk, H. et al. (2002) Cell 108, 247-259.


Entrez-Gene Id 25
Swiss-Prot Acc. P00519

Data Sheets & Documentation


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DELFIA® is a registered trademark of PerkinElmer, Inc.