Upstream / Downstream


Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee


To get local purchase information on this product, click here


Find answers on our FAQs page.


Visit PhosphoSitePlus®

PTM information and tools available.


Product Includes Quantity (with Count) Solution Color
Caspase-7 Ab 1 x 150 µl
Chaps Cell Extract Buffer (10X) 9852 1 x 1.5 ml
Fluorogenic Caspase-7 Substrate 1 x 0.1 ml
1M DTT 1 x 1 ml
10X Reaction Buffer 1 x 15 ml

Product Description

The Caspase-7 Activity Assay Kit includes all reagents necessary to assay caspase-7 activity, including Caspase-7 Antibody, fluorescent caspase substrate, reaction buffer and cell extract buffer. The assay system uses Caspase-7 Antibody to selectively immunoprecipitate caspase-7. Fluorogenic substrate is added and is cleaved proportionally to the amount of activated caspase-7, generating free fluorescent AFC. Free AFC is measured fluorometrically at lmax = 505 nM. Compared to other commercially available caspase-7 activity assays, this kit is designed to be more specific by using Caspase-7 Antibody to selectively immunoprecipitate caspase-7 from cell lysates.

Molecular Formula:

Expected Molecular Weight: 35, 20 kDa

Specificity / Sensitivity

The kit components are designed to measure the endogenous caspase-7 enzymatic activity in human samples. Caspase-7 antibody immunoprecipitates human caspase-7 and is unique to this kit.

Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processsing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5).

1.  Fernandes-Alnemri, T. et al. (1995) Cancer Res. 55, 6045-6052.

2.  Duan, H. et al. (1996) J. Biol. Chem. 271, 1621-1625.

3.  Cohen, G.M. (1997) Biochem J 326 ( Pt 1), 1-16.

4.  Lippke, J. A. et al. (1996) J. Biol. Chem. 271, 1825-1828.

5.  Thornberry, N. A. et al. (1997) J. Biol. Chem. 272, 17907-17911.

Entrez-Gene Id 840
Swiss-Prot Acc. P55210

Data Sheets & Documentation

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.