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Product Includes Quantity (with Count) Solution Color
Caspase-7 Ab 1 x 150 µl
Chaps Cell Extract Buffer (10X) 9852 1 x 1.5 ml
Fluorogenic Caspase-7 Substrate 1 x 0.1 ml
1M DTT 1 x 1 ml
10X Reaction Buffer 1 x 15 ml

Product Description

The Caspase-7 Activity Assay Kit includes all reagents necessary to assay caspase-7 activity, including Caspase-7 Antibody, fluorescent caspase substrate, reaction buffer and cell extract buffer. The assay system uses Caspase-7 Antibody to selectively immunoprecipitate caspase-7. Fluorogenic substrate is added and is cleaved proportionally to the amount of activated caspase-7, generating free fluorescent AFC. Free AFC is measured fluorometrically at lmax = 505 nM. Compared to other commercially available caspase-7 activity assays, this kit is designed to be more specific by using Caspase-7 Antibody to selectively immunoprecipitate caspase-7 from cell lysates.

Molecular Formula:

Expected Molecular Weight: 35, 20 kDa

Specificity / Sensitivity

The kit components are designed to measure the endogenous caspase-7 enzymatic activity in human samples. Caspase-7 antibody immunoprecipitates human caspase-7 and is unique to this kit.

Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processsing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5).

1.  Fernandes-Alnemri, T. et al. (1995) Cancer Res. 55, 6045-6052.

2.  Duan, H. et al. (1996) J. Biol. Chem. 271, 1621-1625.

3.  Cohen, G.M. (1997) Biochem J 326 ( Pt 1), 1-16.

4.  Lippke, J. A. et al. (1996) J. Biol. Chem. 271, 1825-1828.

5.  Thornberry, N. A. et al. (1997) J. Biol. Chem. 272, 17907-17911.

Entrez-Gene Id 840
Swiss-Prot Acc. P55210

Data Sheets & Documentation

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.