Upstream / Downstream

Explore pathways related to this product.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Labor Day. We will reopen on Tuesday, September 2nd.

Thank you for your patience.

To Purchase # 9860S

9860S 1 Kit (125 35mm wells) $209.00
$ 0. 00

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity (with Count)
10X Fixative Solution 15 ml
10X Staining Solution 15 ml
100X Solution A 1.5 ml
100X Solution B 1.5 ml
X-Gal 150 mg

Product Description

The Senescence β-Galactosidase Staining Kit is designed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells not found in presenescent, quiescent or immortal cells (3). The kit includes all reagents necessary for this assay.


Specificity / Sensitivity

The kit detects β-galactosidase activity at pH 6 in cultured cells. β-galactosidase activity at pH 6 is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.


Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable (1). Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition (2). An enlarged cell size, expression of pH-dependent beta-galactosidase activity (3), and an altered pattern of gene expression (4,5) further characterize senescent cells.


1.  Goldstein, S. (1990) Science 249, 1129-1133.

2.  Sherwood, S. W. et al. (1988) Proc. Natl. Acad. Sci. , USA 85, 9086-9090.

3.  Dimri, G. et al. (1995) Proc. Natl. Acad. Sci., USA 92, 9363-9367.

4.  Cristofalo, V. J. et al. (1998) Crit. Rev. Eukaryot Gene Expr. 8, 43-80.

5.  Linskens, M. H. et al. (1995) Nucleic Acid Res. 23, 3244-3251.


Entrez-Gene Id 945006
Swiss-Prot Acc. P00722


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.