Upstream / Downstream
Explore pathways related to this product.
Our U.S. Offices Are Closed
Our U.S. offices are closed due to inclement weather. We will reopen on Wednesday, January 28th.
Thank you for your patience.
To Purchase # 9860S
|9860S||1 Kit (125 35mm wells)||$209.00|
|$ 0. 00|
Find answers on our FAQs page.
PTM information and tools available.
Senescence β-Galactosidase Staining Kit #9860
Gallery: Senescence β-Galactosidase Staining Kit #9860
- β-Galactosidase staining at pH 6 on normal WI38 cells at population doubling 29 (left) and senescent WI38 cells at population doubling 36 (right).
|Product Includes||Quantity (with Count)|
|10X Fixative Solution||15 ml|
|10X Staining Solution||15 ml|
|100X Solution A||1.5 ml|
|100X Solution B||1.5 ml|
The Senescence β-Galactosidase Staining Kit is designed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells not found in presenescent, quiescent or immortal cells (3). The kit includes all reagents necessary for this assay.
The kit detects β-galactosidase activity at pH 6 in cultured cells. β-galactosidase activity at pH 6 is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.
Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable (1). Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition (2). An enlarged cell size, expression of pH-dependent beta-galactosidase activity (3), and an altered pattern of gene expression (4,5) further characterize senescent cells.
Product Specific References
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.