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To Purchase # 11996SC

11996SC 10 µg (With Carrier)
11996SF 10 µg (Carrier Free)
11996LC 50 µg (With Carrier)
11996LF 50 µg (Carrier Free)

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Source / Purification

Recombinant mouse His6IL-25 (mHis6IL-25) Val17-Ala169 (Accession #NP_542767) was expressed in human 293 cells at Cell Signaling Technology.

Product Description

Purity:

>97% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant mHis6IL-25. All lots are greater than 97% pure.


Molecular Formula:

Recombinant N-terminally His6-tagged mIL-25 has a calculated MW of 20,051 Da. DTT-reduced protein migrates as a 24-31 kDa polypeptide. The nonreduced cystine-linked homodimer migrates as a 46-50 kDa protein. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino terminus of recombinant mHis6IL-25 was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant mHis6IL-25 was determined in mouse splenocyte and HT-29 cell cytokine secretion assays. The ED50 of each lot is between 0.5-1.5 ng/ml in mouse splenocyte cells and 0.25-1.5 ng/mL in HT-29 cells.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg mHis6IL-25.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of mHis6IL-25 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg mHis6IL-25. Carrier free: Lyophilized from a 0.22 μm filtered solution of mHis6IL-25 in 20 mM Tris, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-25, also known as IL-17E, is a member of the IL-17 superfamily of cytokines. IL-25 is expressed in epithelial cells, CD4+ T cells, mast cells, and eosinophils (1). Many cell types are responsive to IL-25, including T cells, macrophages, and epithelial cells (1). The receptor for IL-25 consists of a heterodimer of IL-17RA and IL-17RB (1,2). IL-25 promotes Th2 type immune responses by induction of IL-5, IL-4, and IL-13 and may contribute to allergic inflammation and asthma (1-3). IL-25 has also been shown in research to promote Th9 cell activation and induce apoptosis in breast cancer cells (4,5).


1.  Iwakura, Y. et al. (2011) Immunity 34, 149-62.

2.  Rickel, E.A. et al. (2008) J Immunol 181, 4299-310.

3.  Petersen, B.C. et al. (2012) Nat Med 18, 751-8.

4.  Angkasekwinai, P. et al. (2010) Nat Immunol 11, 250-6.

5.  Furuta, S. et al. (2011) Sci Transl Med 3, 78ra31.


Entrez-Gene Id 140806
Swiss-Prot Acc. Q8VHH8


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.