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To Purchase # 12670SC

12670SC 10 µg (With Carrier)
12670SF 10 µg (Carrier Free)
12670LC 50 µg (With Carrier)
12670LF 50 µg (Carrier Free)

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Source / Purification

Recombinant mouse His6IL-21 (mHis6IL-21) Pro25-Ser146 (Accession #NP_068554.1) was expressed in human 293 cells at Cell Signaling Technology.

Product Description

Purity:

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant mHis6IL-21. All lots are greater than 95% pure.


Molecular Formula:

Recombinant His6-tagged IL-21 has a calculated MW of 16,952. DTT-reduced and non-reduced protein migrate as 18-28 kDa polypeptides. Heterogeneity in SDS PAGE is due to glycosylation. The expected amino terminus of recombinant mHis6IL-21 was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant mHis6IL-21 was determined in a B9 cell proliferation assay. The ED50 of each lot is between and 20-80 pg/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg mHis6IL-21.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered 10 mM HEPES pH 7.0 solution containing 20 μg BSA per 1 μg mHis6IL-21. Carrier free: Lyophilized from a 0.22 μm filtered 10 mM HEPES pH 7.0 solution.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-21 is a pleiotropic type I cytokine that is produced by CD4+ T cells, Th17 cells and NKT cells (1,2). The IL-21 receptor is a heterodimer that consists of an IL-21R and the common γ chain, γc (1). IL-21/IL-21R signaling involves activation of JAKs1/3, STAT3 and, to a lesser extent, STAT5 and STAT1 (1,2). IL-21 promotes B cell proliferation, survival and differentiation into plasma cells (3,4). IL-21 activates the cytolytic functions of CD8+ T cells and NK cells, and has been shown in mouse models to play a key role in immunity to chronic viral infections and promote anti-tumor immunity (5-7).


1.  Leonard, W.J. et al. (2008) J Leukoc Biol 84, 348-56.

2.  Spolski, R. and Leonard, W.J. (2008) Curr Opin Immunol 20, 295-301.

3.  Parrish-Novak, J. et al. (2000) Nature 408, 57-63.

4.  Parrish-Novak, J. et al. (2002) J Leukoc Biol 72, 856-63.

5.  Pan, X.C. et al. (2013) Oncol Lett 5, 90-96.

6.  Fröhlich, A. et al. (2009) Science 324, 1576-80.

7.  Yi, J.S. et al. (2009) Science 324, 1572-6.


Entrez-Gene Id 60505
Swiss-Prot Acc. Q9ES17


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.