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To Purchase # 5183SC

5183SC 10 µg (With Carrier)
5183SF 10 µg (Carrier Free)
5183LC 50 µg (With Carrier)
5183LF 50 µg (Carrier Free)

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Source / Purification

Recombinant human IL-29 (hIL-29) Gly20-Thr200 (Accession #NP_742152) was expressed in human 293 cells at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-29. All lots are greater than 98% pure.


Molecular Formula:

Recombinant hIL-29 contains no "tags" and the nonglycosylated protein has a calculated MW of 20,018. DTT-reduced and non-reduced protein migrate as 31 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal GPVPT of recombinant hIL-29 was verified by amino acid sequencing.


Bioactivity:

The bioactivity of hIL-29 was determined in a virus protection assay. The ED50 of each lot is between 0.40-1.45 ng/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1μg hIL-29.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-29. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-29 is a member of the IFNλ family of cytokines and shares many functions with the type I family of interferons (1,2). IL-29 is produced by monocytes and dendritic cells in response to viral infection and stimulation with toll-like receptor ligands (1-3). IL-29 is a potent antiviral cytokine. The antiviral activities of IL-29 include the upregulation of MHC Class I expression on the cell surface, and the expression of PKR, MxA, and 2’, 5'-OAS (1,2). The IL-29 receptor consists of IL-28Rα and IL-10Rβ is expressed on most non-hematopoietic cells (3). IL-29 binding induces the phosphorylation of Stats 1, 2 and 3 (1,3).


1.  Kotenko, S.V. et al. (2003) Nat Immunol 4, 69-77.

2.  Sheppard, P. et al. (2003) Nat Immunol 4, 63-8.

3.  Donnelly, R.P. et al. (2004) J Leukoc Biol 76, 314-21.


Entrez-Gene Id 282618
Swiss-Prot Acc. Q8IU54

Product Specific References

Voigt, E. et al. (2013) Virol J 10, 224.


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.