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To Purchase # 5224SC

5224SC 10 µg (With Carrier) $199.00
5224SF 10 µg (Carrier Free) $199.00
5224LC 50 µg (With Carrier) $599.00
5224LF 50 µg (Carrier Free) $599.00
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Source / Purification

Recombinant mouse IL-22 (mIL-22) Leu34-Val179 (Accession #NP_058667) was expressed in human 293 cells at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-22. All lots are greater than 98% pure.


Molecular Formula:

Recombinant mIL-22 contains no "tags" and the nonglycosylated protein has a calculated MW of 16,640. DTT-reduced and non-reduced protein migrate as 31 kDa polypeptides. Lower mobility and heterogeneity in SDS PAGE are due to glycosylation. The expected amino-terminal LPVNT of recombinant mIL-22 was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant mIL-22 was determined by its ability to induce IL-10 production by COLO 205 cells. The ED50 of each lot is between 0.2-0.8 ng/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1μg mIL-22.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-22. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-22, a member of the IL-10 family (1,2), is expressed by Th17 CD4+ T cells, activated T cells, Th1 cells and NK cells (3). Expression of IL-22 in combination with a select group of cytokines defines a subset of Th cells(4). IL-22 induces proinflammatory responses, drives production of antimicrobial peptides, and is involved in tissue-repair and wound-healing responses (1). The IL-22 receptor is a heterodimer of IL-22R1 and IL-10R2 (5). Expression of IL-22R is restricted to tissue-resident cells, particularly those of epithelial origin, whereas the IL-10R2 chain is expressed in many more cell types. IL-22 induces phosphorylation of Jak1 and Tyk2, leading to activation of Stat3 and, to a lesser extent, Stat1 and Stat5 (1). IL-22 responses can involve activation of the MEK-ERK-RSK, JNK-SAPK, and p38 pathways (1). Elevated levels of IL-22 have been associated with Crohn's disease and rheumatoid arthritis. IL-22 plays an essential role in host response to the pulmonary pathogen Klebsiella pneumoniae (6).


1.  Ouyang, W. et al. (2008) Immunity 28, 454-67.

2.  Dumoutier, L. et al. (2000) Proc Natl Acad Sci U S A 97, 10144-9.

3.  Takatori, H. et al. (2008) Mod Rheumatol 18, 533-41.

4.  Eyerich, S. et al. (2009) J Clin Invest 119, 3573-85.

5.  Nagalakshmi, M.L. et al. (2004) Int Immunopharmacol 4, 679-91.

6.  Aujla, S.J. et al. (2008) Nat Med 14, 275-81.


Entrez-Gene Id 50929
Swiss-Prot Acc. Q9JJY9


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.