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To Purchase # 5236SC

5236SC 10 µg (With Carrier)
5236SF 10 µg (Carrier Free)

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Source / Purification

Recombinant human IL-1α (hIL-1α) Ser113-Ala271 (Accession #NP_000566) was produced in E. coli at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-1α. All lots are greater than 98% pure.


Molecular Formula:

Recombinant hIL-1α does not have a Met on the amino terminus and has a calculated MW of 18,063. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal SSPFS of recombinant hIL-1α was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant hIL-1α was determined by its ability to induce IL-8 production by primary human fibroblasts. The ED50 of each lot is between 5-15 pg/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg hIL-1α.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 4mM DTT and 20 μg BSA per 1 μg hIL-1α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 4mM DTT.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-1α is a pro-inflammatory cytokine produced by activated monocytes, lymphocytes, and epithelial cells (1). IL-1α is synthesized as an active precursor protein that appears to be cleaved by cytosolic proteases into its mature form (1,2). Often, precursor and mature forms of IL-1α are primarily retained intracellularly rather than constitutively secreted. (1,2). Signaling by IL-1α involves IL-1α binding to an IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling occurs through activation of MAP kinase and NF-κB pathways (1,2). IL-1α also binds to an IL-RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1α activity is inhibited through IL-1R antagonist (IL-1Ra) that binds IL-1R1 but does not signal. IL-1α has been shown to be a key mediator of virus-induced inflammatory responses in mice (3).


1.  Dinarello, C.A. (1996) Blood 87, 2095-147.

2.  Allan, S.M. et al. (2005) Nat Rev Immunol 5, 629-40.

3.  Di Paolo, N.C. et al. (2009) Immunity 31, 110-21.


Entrez-Gene Id 3552
Swiss-Prot Acc. P01583


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.