Upstream / Downstream
Explore pathways related to this product.
Our U.S. Offices Are Closed
Our U.S. offices are closed in observance of Labor Day. We will reopen on Tuesday, September 2nd.
Thank you for your patience.
To Purchase # 5717SC
|5717SC||10 µg (With Carrier)||$199.00|
|5717SF||10 µg (Carrier Free)||$199.00|
|5717LC||50 µg (With Carrier)||$599.00|
|5717LF||50 µg (Carrier Free)||$599.00|
|$ 0. 00|
Find answers on our FAQs page.
PTM information and tools available.
Human Cystatin C (hCystatin C) #5717
The purity of recombinant hCystatin C was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hCystatin C and staining overnight with Coomassie Blue.Learn more about how we get our images
Gallery: Human Cystatin C (hCystatin C) #5717
- The purity of recombinant hCystatin C was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hCystatin C and staining overnight with Coomassie Blue.
- The activity of hCystatin C was determined by assessing its ability to inhibit papain-mediated cleavage of a fluorogenic subtrate, Z-Phe-Arg-AMC.
Recombinant human Cystatin C (hCystatin C) Ser27-Ala146 (Accession #NP_000090) was expressed in human 293 cells at Cell Signaling Technology.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hCystatin C. All lots are greater than 98% pure.Molecular Formula:
Recombinant hCystatin C does not have a Met on the amino terminus and has a calculated MW of 13,347. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal SSPGK of recombinant hCystatin C was verified by amino acid sequencing.Bioactivity:
The activity of hCystatin C was determined by assessing its ability to inhibit papain-mediated cleavage of a fluorogenic subtrate. The ED50 of each lot 1-12 nM.Endotoxin:
Less than 0.01 ng endotoxin/1μg hCystatin C.
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hCystatin C. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Cystatin C is a 14 kDa member of the Cystatin superfamily of cysteine protease inhibitors (1). Most cell types secrete Cystatin C (1). Cystatin C inhibits cathepsins, and thereby may function as a tumor suppressor by inhibiting cathepsin mediated tumor cell invasion (2). In addition, this tumor suppressor function can also be attributed to Cystatin C's ability to antagonize TGF-β1 signaling (2,3). Cystatin C may also modulate antigen presentation through its ability to inhibit cathepsins (4). Cystatin C is also a biomarker for kidney dysfunction (5).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.