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To Purchase # 5725SC

5725SC 10 µg (With Carrier)
5725SF 10 µg (Carrier Free)

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Source / Purification

Recombinant mouse His6IL-27 (mHis6IL-27) is a heterodimer that is composed of EBI3 Tyr19-Pro228 (Accession# NP_056581) linked to p28 Phe29-Ser234 (Accession# NP_663611) via the linker GGGSGGGSGGGSGGGS. mHis6IL-27 was expressed in human 293 cells at Cell Signaling Technology.

Product Description

Purity:

>97% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mHis6IL-27. All lots are greater than 97% pure.


Molecular Formula:

Recombinant N-terminally His6-tagged mIL-27 has a calculated MW of 50,551. DTT-reduced and non-reduced protein migrate as 55 kDa polypeptides. The expected amino terminus of recombinant mHis6IL-27 was verified by amino acid sequencing.


Bioactivity:

The bioactivity of mHis6IL-27 was determined in a virus protection assay. The ED50 of each lot is between 1-6 ng/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1μg mHis6IL-27.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mHis6IL-27. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-27 is a heterodimer that consists of p28 and EBI3 (1). Antigen presenting cells including monocytes, macrophages, and dendritic cells are the primary sources of IL-27 (1). IL-27 is a pleiotropic cytokine with both pro- and anti-inflammatory activities. IL-27 suppresses TH17 responses and promotes type I regulatory cell differentiation (2-4). In contrast, IL-27 has been shown to play a pro-inflammatory role in a mouse model of T cell-induced colitis (5). IL-27 may also have anti-viral activities (6). IL-27 exerts its effects through a heterodimeric receptor consisting of WSX-1 and gp130 (1,7). IL-27-induced signaling results in Stat1 and Stat3 phosphorylation (1,7).


1.  Pflanz, S. et al. (2002) Immunity 16, 779-90.

2.  Villarino, A.V. et al. (2010) J Immunol 185, 6461-71.

3.  Apetoh, L. et al. (2010) Nat Immunol 11, 854-61.

4.  Murugaiyan, G. et al. (2010) Proc Natl Acad Sci U S A 107, 11495-500.

5.  Cox, J.H. et al. (2011) J Exp Med 208, 115-23.

6.  Bender, H. et al. (2009) Hepatology 50, 585-91.

7.  Pflanz, S. et al. (2004) J Immunol 172, 2225-31.


Entrez-Gene Id 246779
Swiss-Prot Acc. Q8K3I6


For Research Use Only. Not For Use In Diagnostic Procedures.
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