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To Purchase # 8460SC

8460SC 10 µg (With Carrier)
8460SF 10 µg (Carrier Free)
8460LC 50 µg (With Carrier)
8460LF 50 µg (Carrier Free)

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Source / Purification

Recombinant human His64-1BBL (hHis64-1BBL) Arg71-Glu254 (Accession #NP_003802) was expressed in human 293 cells at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hHis64-1BBL. All lots are greater than 98% pure.


Molecular Formula:

Recombinant N-terminally His6-tagged h4-1BBL has a calculated MW of 22,020. DTT-reduced and non-reduced protein migrate as 22 kDa polypeptides. The expected amino terminus of recombinant hHis64-1BBL was verified by amino acid sequencing.


Bioactivity:

The activity of hHis64-1BBL was assessed by its ability to bind to 4-1BB in a functional ELISA. The concentration at which half-maximal binding was observed for each lot was 6-18 ng/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg hHis64-1BBL.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hHis64-1BBL. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

4-1BBL (also known as TNFSF9) is a member of the TNF-R superfamily, which includes OX40L, CD27L, CD40L, and GITRL (1). 4-1BBL is expressed primarily on antigen presenting cells, such as macrophages, dendritic cells, and B cells (2). 4-1BBL binds to 4-1BB on the surface of activated T cells (1). 4-1BBL/4-1BB binding activates the NF-κB pathway via TRAF1, 2, and 3 (1). 4-1BBL functions as a co-stimulatory molecule, inducing T cell proliferation, cytolytic activity, and cytokine production (2). Matrix metalloproteases can generate soluble 4-1BBL by cleaving it from the cell surface (3). The in vitro activity of soluble 4-1BBL is enhanced by cross-linking (4,5).


1.  Vinay, D.S. and Kwon, B.S. (2009) Cell Biol Int 33, 453-65.

2.  Wen, T. et al. (2002) J Immunol 168, 4897-906.

3.  Salih, H.R. et al. (2001) J Immunol 167, 4059-66.

4.  Rabu, C. et al. (2005) J Biol Chem 280, 41472-81.

5.  Wyzgol, A. et al. (2009) J Immunol 183, 1851-61.


Entrez-Gene Id 8744
Swiss-Prot Acc. P41273


For Research Use Only. Not For Use In Diagnostic Procedures.
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