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To Purchase # 8796SC

8796SC 10 µg (With Carrier)
8796SF 10 µg (Carrier Free)
8796LC 50 µg (With Carrier)
8796LF 50 µg (Carrier Free)

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Source / Purification

Recombinant human IL-28B (hIL-28B) Arg26-Val196 (Accession #NP_742151) was expressed in human 293 cells at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-28B. All lots are greater than 98% pure.


Molecular Formula:

Recombinant hIL-28B contains no "tags" and the nonglycosylated protein has a calculated MW of 19,264. DTT-reduced and non-reduced protein migrate as 21 kDa polypeptides with non-reduced protein having slightly greater mobility due to intramolecular cystines. The expected amino-terminal RLRGA of recombinant hIL-28B was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant hIL-28B was determined in a virus protection assay. The ED50 of each lot is between 0.4-9 ng/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg hIL-28B.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-28B. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-28B is a member of the Interferon λ family of cytokines, which includes IL-29 and IL-28A (1). IL-28B is produced by a number of cell types and shares many functions with Type I Interferons (1-3). IL-28B exhibits anti-viral activities in vitro and in vivo (1,2). Investigators have found that IL-28B directly inhibits tumor cell proliferation and promotes anti-tumor immune responses in vivo (1,3). The IL-28 receptor is a heterodimer of the IL-28Rα and IL-10RI (1). IL-28B activates Stat1, Stat3, and Stat5 (1). IL-28Rα expression is limited to a few cell types, including plasmacytoid DC and epithelial cells (1).


1.  Li, M. et al. (2009) J Leukoc Biol 86, 23-32.

2.  Ank, N. et al. (2006) J Virol 80, 4501-9.

3.  Numasaki, M. et al. (2007) J Immunol 178, 5086-98.


Entrez-Gene Id 282617
Swiss-Prot Acc. Q8IZI9

Product Specific References

Voigt, E. et al. (2013) Virol J 10, 224.


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.