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To Purchase # 8920SC

8920SC 10 µg (With Carrier)
8920SF 10 µg (Carrier Free)
8920LC 50 µg (With Carrier)
8920LF 50 µg (Carrier Free)

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Source / Purification

Recombinant human IL-21 (hIL-21) Gln30-Ser155 (Accession # NM_021803) was expressed in human 293 cells at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-21. Less than 9% is nicked after Arg114 to generate 6 kDa and 10 kDa polypeptides observed in reduced (+) hIL-21.


Molecular Formula:

Recombinant hIL-21 contains no "tags" and the nonglycosylated protein has a calculated MW of 15,463. DTT-reduced and non-reduced protein migrate as 16 kDa polypeptides, with non-reduced having slightly greater mobility due to intramolecular cystines. The expected amino-terminal QGQDR of recombinant hIL-21 was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant hIL-21 was determined by its ability to induce IFN-γ production by NK-92 cells. The ED50 of each lot is between 1.0-6.0 ng/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg hIL-21.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-21. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-21 is produced by Th17, T follicular helper (Tfh) and NKT cells (1-3). Targets include T cells, B cells, NK cells and dendritic cells (1-3,5). IL-21 induces proliferation and activation of NK cells, thereby up-regulating IFN-γ production and cytotoxic activity (1,3,4). IL-21 increases proliferation and survival of CD40 primed B cells (1,2) and appears to have a significant role in plasma cell differentiation (2). IL-21 binds to a complex consisting of IL-21Rα and the common γ chain, γc. IL-21 binding activates the Jak1/Jak3, Stat1, Stat3 and Stat5 pathway. IL-21 binding can also activate the MAP kinase and PI3K/Akt pathways.


1.  Parrish-Novak, J. et al. (2000) Nature 408, 57-63.

2.  Konforte, D. et al. (2009) J Immunol 182, 1781-7.

3.  Parrish-Novak, J. et al. (2002) J Leukoc Biol 72, 856-63.

4.  Strengell, M. et al. (2002) J Immunol 169, 3600-5.

5.  Davis, I.D. et al. (2007) Clin Cancer Res 13, 6926-32.


Entrez-Gene Id 59067
Swiss-Prot Acc. Q9HBE4


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.