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To Purchase # 8923SC

8923SC 10 µg (With Carrier)
8923SF 10 µg (Carrier Free)
8923LC 50 µg (With Carrier)
8923LF 50 µg (Carrier Free)

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Source / Purification

Recombinant mouse interleukin-3 (mIL-3) Ala27-Cys166 (Accession # NM_010556) was produced in E. coli at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-3. All lots are greater than 98% pure.


Molecular Formula:

Recombinant mIL-3 does not have a Met on the amino terminus and has a calculated MW of 15,674. DTT-reduced protein migrates as a 16 kDa polypeptide and non-reduced protein migrates as a 14 kDa polypeptide due to intramolecular cystines. The expected amino-terminal ASISG of recombinant mIL-3 was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant mIL-3 was determined in MC/9 and BaF3 cell proliferation assays. The ED50 of each lot is between 2-50 pg/ml in MC/9 cells and 20-90 pg/mL in BaF3 cells.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg mIL-3.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-3. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-3 is produced by T cells, mast cells and eosinophils (1). Target cells include hematopoietic progenitors, neutrophils, macrophages, mast cells, eosinophils, lymphoid and erythroid cells (1). IL-3 supports growth and differentiation and is used as a media additive to support culture of many cell types (1). The IL-3 receptor is a heterodimer of the IL-3 specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. (1). Binding of IL-3 can also involve substitution of the βc by a βIL-3-chain that appears to be specific for IL-3 (1,2). Binding of IL-3 to its cognate receptor(s) induces activation of Jak2, phosphorylation of multiple Stats (1,3,5,6), and the PI3K/Akt pathway (1). IL-3 may play an important role in the development of airway inflammation associated with asthma (3,4,5).


1.  Reddy, E.P. et al. (2000) Oncogene 19, 2532-47.

2.  Hara, T. and Miyajima, A. (1992) EMBO J 11, 1875-84.

3.  Asquith, K.L. et al. (2008) J Immunol 180, 1199-206.

4.  Schroeder, J.T. et al. (2009) J Immunol 182, 2432-8.

5.  Munitz, A. et al. (2006) J Immunol 177, 77-83.


Entrez-Gene Id 16187
Swiss-Prot Acc. P01586


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.