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To Purchase # 8930SC

8930SC 10 µg (With Carrier)
8930SF 10 µg (Carrier Free)
8930LC 50 µg (With Carrier)
8930LF 50 µg (Carrier Free)

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Source / Purification

Recombinant human G-CSF (hG-CSF) Thr31-Pro204 (Accession #NP_757373) was expressed in human 293 cells at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hG-CSF. All lots are greater than 98% pure.


Molecular Formula:

Recombinant hG-CSF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,986. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal TPLGP of recombinant hG-CSF was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant hG-CSF was determined in a M-NFS-60 cell proliferation assay. The ED50 of each lot is between 20-150 pg/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1μg hG-CSF.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of 40 mM phosphate pH 4.0 containing 250 mM NaCl and 20 μg BSA per 1 μg hG-CSF. Carrier free: Lyophilized from a 0.22 μm filtered solution of 40 mM phosphate pH 4.0 containing 250 mM NaCl.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

G-CSF is a hematopoietic cytokine essential for neutrophil development, survival, and egress from bone marrow (1-4). Macrophages and monocytes are the predominant producers of G-CSF (3) and endothelial cells, fibroblasts and neuronal cells can produce G-CSF in response to inflammatory stimuli (3). G-CSF inhibits apoptosis in neutrophils and neurons (4,5). G-CSF stimulates proliferation and differentiation of neuronal progenitor cells (5). G-CSF binding to G-CSFR induces receptor dimerization and activation of Jak1/2 tyrosine phosphorylation (3,6). Signaling is through Stat3, ERK, p38, and Akt (5,6). Absence of functional G-CSF or its receptor in humans and mice causes neutropenia (7,8).


1.  Furze, R.C. and Rankin, S.M. (2008) Immunology 125, 281-8.

2.  Demetri, G.D. and Griffin, J.D. (1991) Blood 78, 2791-808.

3.  Srinivasa, S.P. and Doshi, P.D. (2002) Leukemia 16, 244-53.

4.  van Raam, B.J. et al. (2008) Blood 112, 2046-54.

5.  Schneider, A. et al. (2005) J Clin Invest 115, 2083-98.

6.  Nicholson, S.E. et al. (1994) Proc Natl Acad Sci U S A 91, 2985-8.

7.  Lieschke, G.J. et al. (1994) Blood 84, 1737-46.

8.  Dong, F. et al. (1994) Proc Natl Acad Sci U S A 91, 4480-4.


Entrez-Gene Id 1440
Swiss-Prot Acc. P09919


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.