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To Purchase # 8931SC

8931SC 10 µg (With Carrier) $199.00
8931SF 10 µg (Carrier Free) $199.00
8931LC 50 µg (With Carrier) $599.00
8931LF 50 µg (Carrier Free) $599.00
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Source / Purification

Recombinant human IL-22 (hIL-22) Ala34-Ile179 (Accession #NP_065386) was expressed in human 293 cells at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-22. All lots are greater than 98% pure.


Molecular Formula:

Recombinant hIL-22 contains no "tags" and the nonglycosylated protein has a calculated MW of 16,749. DTT-reduced and non-reduced protein migrate as 34 kDa polypeptides. Lower mobility and heterogeneity in SDS PAGE are due to glycosylation. The expected amino-terminal APISS of recombinant hIL-22 was verified by amino acid sequencing.


Bioactivity:

The bioactivity of recombinant IL-22 was determined by its ability to induce IL-10 production by COLO 205 cells. The ED50 of each lot is between 100-300 pg/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1μg hIL-22.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-22. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-22, a member of the IL-10 family (1,2), is expressed by Th17 CD4+ T cells, activated T cells, Th1 cells and NK cells (3). IL-22 induces proinflammatory responses, drives production of antimicrobial peptides, and is involved in tissue-repair and wound-healing responses (1). The IL-22 receptor is a heterodimer of IL-22R1 and IL-10R2 (4). Expression of IL-22R is restricted to tissue-resident cells, particularly those of epithelial origin, whereas the IL-10R2 chain is expressed in many more cell types. IL-22 induces phosphorylation of Jak1 and Tyk2, leading to activation of Stat3 and, to a lesser extent, Stat1 and Stat5 (1). IL-22 responses can involve activation of the MEK-ERK-RSK, JNK-SAPK, and p38 pathways (1). Elevated levels of IL-22 have been associated with Crohn's disease and rheumatoid arthritis. IL-22 plays an essential role in host response to the pulmonary pathogen Klebsiella pneumoniae (5).


1.  Ouyang, W. et al. (2008) Immunity 28, 454-67.

2.  Dumoutier, L. et al. (2000) Proc Natl Acad Sci U S A 97, 10144-9.

3.  Takatori, H. et al. (2008) Mod Rheumatol 18, 533-41.

4.  Nagalakshmi, M.L. et al. (2004) Int Immunopharmacol 4, 679-91.

5.  Aujla, S.J. et al. (2008) Nat Med 14, 275-81.


Entrez-Gene Id 50616
Swiss-Prot Acc. Q9GZX6


For Research Use Only. Not For Use In Diagnostic Procedures.
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