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To Purchase # 9584SC

9584SC 10 µg (With Carrier)
9584SF 10 µg (Carrier Free)
9584LC 50 µg (With Carrier)
9584LF 50 µg (Carrier Free)

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Source / Purification

Recombinant mouse IL-17B (mIL-17B) His21-Phe180 (Accession #NP_062381) was produced in E. coli at Cell Signaling Technology.

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-17B. All lots are greater than 98% pure.


Molecular Formula:

Recombinant mIL-17B has a Met on the amino terminus and has a calculated MW of 18,186. DTT-reduced and non-reduced protein migrate as 19 kDa polypeptides. The expected amino-terminal MHPRN of recombinant mIL-17B was verified by amino acid sequencing.


Bioactivity:

The activity of mIL-17B was assessed by its ability to bind to IL-17RB in a functional ELISA. The concentration at which half-maximal binding was observed for each lot was 0.1-1.0 µg/ml.


Endotoxin:

Less than 0.01 ng endotoxin/1 μg mIL-17B.


Product Usage Information

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-17B. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.


Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-17B is a member of the IL-17 family of structurally related proteins (1). Unlike other IL-17 family members, IL-17B is a non-covalently linked homodimer (1,2). IL-17B is expressed by chondrocytes and neurons, and binds to the IL-17RB receptor (1-4). Human IL-17B has been shown to induce TNF-α and IL-1β from monocytic THP-1 cells and may be involved in neutrophil recruitment in vivo (3). However, the exact biological functions of IL-17B remain elusive.


1.  Iwakura, Y. et al. (2011) Immunity 34, 149-62.

2.  Shi, Y. et al. (2000) J Biol Chem 275, 19167-76.

3.  Moore, E.E. et al. (2002) Neuromuscul Disord 12, 141-50.

4.  Li, H. et al. (2000) Proc Natl Acad Sci U S A 97, 773-8.


Entrez-Gene Id 56069
Swiss-Prot Acc. Q9QXT6


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.