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REACTIVITY
Product Includes Volume Solution Color
Phospho-FRA1(Ser265) Rabbit mAb Coated Microwells 96 tests
FRA1 Rabbit Detection mAb (Biotinylated) 11 ml Green
Streptavidin-HRP 11 ml Red
Sealing Tape 2 sheets

Product Description

The PathScan® Phospho-FRA1 (Ser265) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of FRA1 when phosphorylated at Ser265. A Phospho-FRA1 (Ser265) Rabbit mAb has been coated onto the microwells. After incubation with cell lysates, phosphorylated FRA1 protein is captured by the coated antibody. Following extensive washing, a biotinylated FRA1 Rabbit Detection mAb is added to detect the captured phospho-FRA1 (Ser265) protein. HRP-linked streptavidin is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of FRA1 phosphorylated at Ser265.

Antibodies in kit are custom formulations specific to kit.


Specificity / Sensitivity

PathScan® Phospho-FRA1 (Ser265) Sandwich ELISA Kit recognizes endogenous levels of FRA1 protein when phosphorylated at Ser265, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), which lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer, and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3).


1.  Tulchinsky, E. (2000) Histol Histopathol 15, 921-8.

2.  Dobrazanski, P. et al. (1991) Mol Cell Biol 11, 5470-8.

3.  Nakabeppu, Y. and Nathans, D. (1991) Cell 64, 751-9.

4.  Rosenberger, S.F. et al. (1999) J Biol Chem 274, 1124-30.

5.  Sasaki, T. et al. (2006) Mol Cell 24, 63-75.

6.  Basbous, J. et al. (2007) Mol Cell Biol 27, 3936-50.

7.  Kovary, K. and Bravo, R. (1991) Mol Cell Biol 11, 2451-9.

8.  Kovary, K. and Bravo, R. (1992) Mol Cell Biol 12, 5015-23.


Entrez-Gene Id 8061
Swiss-Prot Acc. P15407


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.