Upstream / Downstream

Explore pathways related to this product.

Important Ordering Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

REACTIVITY
H
Product Includes Volume Solution Color
hIL-17A Rabbit mAb Coated Microwells 96 tests
Human IL-17A Standard (Lyophilized) 1 vial
SignalKine™ Assay Diluent A01 1.5 ml Colorless
SignalKine™ Sample Diluent S04 25 ml Brown
hIL-17A Detection Rabbit mAb (Biotinylated) (Lyophilized) 100X 1 vial
Detection Antibody Diluent 6 ml Green
HRP-linked Streptavidin (Lyophilized) 100X 1 vial
HRP Diluent 6 ml Red
ELISA Wash Buffer (20X) 25 ml Colorless
Luminol/Enhancer Solution 3 ml Colorless
Stable Peroxide Buffer 3 ml Colorless
Sealing Tape 2 sheets

Product Description

SignalKine™ Human IL-17A Chemiluminescent Sandwich ELISA Kit from Cell Signaling Technology (CST) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human IL-17A (hIL-17A) in multiple matrices. Unknown samples being tested for hIL-17A and hIL-17A standards are added to low volume microwells, where the hIL-17A is captured by the coated hIL-17A Rabbit mAb. Following a washing step, a biotinylated hIL-17A Detection Rabbit mAb is added to detect the captured hIL-17A. HRP-linked Streptavidin is then used for detection of the biotinylated hIL-17A Detection Rabbit mAb. A chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU) is proportional to the quantity of human hIL-17A in the sample.

SignalKine™ Human IL-17A Chemiluminescent Sandwich ELISA Kit detects hIL-17A in multiple matrices that can be quantified by generating a standard curve with the recombinant hIL-17A protein standard provided. The hIL-17A standard range is from 2 to 1500 pg/ml. Samples containing higher levels of hIL-17A can be diluted to fit into the standard range.


Specificity / Sensitivity

Matrices Tested: This ELISA has been validated in cell culture supernatants, plasma (citrate), plasma (EDTA), plasma (heparin), and serum.


Species Reactivity: Human

IL-17A is a cystine-linked homodimeric pro-inflammatory cytokine produced by Th17 cells, a distinct CD4+ T cell lineage (1,2). IL-17A stimulates the production of the pro-inflammatory cytokines IL-1β, TNF-α, and IL-6. IL-17A also induces production of the neutrophil chemoattractants IL-8, CXCL1, and CXCL6, thereby bridging adaptive and innate immunity (1,2). IL-17A is intimately involved in mucosal immunity against bacterial infections (1,3) and has a putative role in some autoimmune disorders (1,4). IL-17A effects appear to be exerted primarily through binding to the IL-17RA (5). IL-17A binding induces production of cytokines, chemokines, and other proteins through activation of the Erk1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways (3,4,6).


1.  Kolls, J.K. and Lindén, A. (2004) Immunity 21, 467-76.

2.  Liang, S.C. et al. (2006) J Exp Med 203, 2271-9.

3.  Dubin, P.J. and Kolls, J.K. (2008) Immunol Rev 226, 160-71.

4.  Zrioual, S. et al. (2009) J Immunol 182, 3112-20.

5.  Wright, J.F. et al. (2008) J Immunol 181, 2799-805.

6.  Rahman, M.S. et al. (2006) J Immunol 177, 4064-71.


Entrez-Gene Id 3605
Swiss-Prot Acc. Q16552


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.