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13336C 1 Kit (96 assays)

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REACTIVITY
Product Includes Volume Solution Color
Total IRAK4 Rabbit Antibody Coated Microwells 96 tests
Total IRAK4 Mouse Monoclonal Antibody 1 Ea Green (Lyophilized)
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) 1 Ea Red (Lyophilized)
Detection Antibody Diluent 11 ml Green
HRP Diluent 11 ml Red
TMB Substrate 7004 11 ml Colorless
STOP Solution 7002 11 ml Colorless
Sealing Tape 2 sheets
ELISA Wash Buffer (20X) 25 ml Colorless
ELISA Sample Diluent 25 ml Blue
Cell Lysis Buffer (10X) 9803 15 ml Yellowish

Product Description

PathScan® Total IRAK4 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total IRAK4 protein. An IRAK4 specific antibody has been coated onto the microwells. After incubation with cell lysates, IRAK4 protein is captured by the coated antibody. Following extensive washing, an IRAK4 mouse mAb is added to detect the captured IRAK4 protein. Anti-Mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of total IRAK4 protein.


Specificity / Sensitivity

PathScan® Total IRAK4 Sandwich ELISA Kit recognizes endogenous levels of IRAK4 protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3).


1.  Dinarello, C.A. (1996) Blood 87, 2095-147.

2.  Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84.

3.  Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.


Entrez-Gene Id 51135
Swiss-Prot Acc. Q9NWZ3


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PathScan® is a trademark of Cell Signaling Technology, Inc.