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Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

REACTIVITY
Product Includes Volume Cap Color
IRS-1 Capture Ab (100X) 0.4 ml Pink
P-IRS-1 (S302) Detection Ab (100X) 0.4 ml Blue
Anti-rabbit IgG, HRP-linked Antibody (1000X) 0.04 ml Red

Product Description

CST's PathScan® Phospho-IRS-1 (Ser302) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-IRS-1 (Ser302) Sandwich ELISA Kit #7283. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IRS-1 Mouse Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysate is added followed by a Phospho-IRS-1 (Ser302) Rabbit Detection Antibody and an HRP-conjugated Anti-Rabbit IgG Antibody. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-1 phosphorylated at Ser302.

*Antibodies in this kit are custom formulations specific to the kit.


Product Usage Information

Antibody pairs have been optimized using recommended buffers, reagents, plates and the included protocol. Solutions should be made fresh daily.


Storage: Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).


1.  Sun, X.J. et al. (1991) Nature 352, 73-7.

2.  Sun, X.J. et al. (1992) J Biol Chem 267, 22662-72.

3.  Myers, M.G. et al. (1993) Endocrinology 132, 1421-30.

4.  Wang, L.M. et al. (1993) Science 261, 1591-4.

5.  Rui, L. et al. (2001) J Clin Invest 107, 181-9.

6.  Gao, Z. et al. (2002) J Biol Chem 277, 48115-21.

7.  Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447.

8.  Ozes, O.N. et al. (2001) Proc Natl Acad Sci U S A 98, 4640-5.

9.  De Fea, K. and Roth, R.A. (1997) Biochemistry 36, 12939-47.

10.  Li, Y. et al. (2004) J Biol Chem 279, 45304-7.


Entrez-Gene Id 3667
Swiss-Prot Acc. P35568


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

7284
PathScan® Phospho-IRS-1 (Ser302) Sandwich ELISA Antibody Pair