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REACTIVITY
Product Includes Volume Solution Color
PDGF Receptor α Rabbit Ab coated microwells 96 tests
Biotinylated PDGF Receptor α Rabbit Detection mAb 11 ml Green
HRP-linked Streptavidin 11 ml Red
TMB Substrate 7004 11 ml Colorless
STOP Solution 7002 11 ml Colorless
ELISA Wash Buffer (20X) 25 ml Colorless
ELISA Sample Diluent 25 ml Blue
Sealing Tape 2 sheets
Cell Lysis Buffer (10X) 9803 15 ml Yellowish

Product Description

CST's PathScan® Total PDGF Receptor α Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total PDGF receptor α protein. A PDGF receptor α Rabbit Antibody has been coated onto the microwells. After incubation with cell lysates, both phospho and nonphospho PDGF receptor α proteins are captured by the coated antibody. Following extensive washing, Biotinylated PDGF Receptor α Rabbit mAb is added to detect both the captured phospho and nonphospho PDGF receptor α protein. HRP-linked Streptavidin is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of total PDGF receptor α protein.

Antibodies in kit are custom formulations specific to kit.


Specificity / Sensitivity

CST's PathScan® Total PDGF Receptor α Sandwich ELISA Kit #7318 detects endogenous levels of total PDGF receptor α protein. As shown in Figure 1, the level of total PDGF receptor α (phospho or nonphospho) before or after treatment of stimulator, remains unchanged as shown by Western analysis or by PathScan® Total PDGF Receptor α Sandwich ELISA Kit #7318. In Figure 3, Western blot analysis of protein captured in the PDGF Receptor α Mouse mAb coated microwell shows major bands corresponding to the PDGF receptor α protein. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


Species Reactivity: Human

Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ-mediated PI3 kinase activation (8).


1.  Deuel, T.F. et al. (1988) Biofactors 1, 213-7.

2.  Bergsten, E. et al. (2001) Nat Cell Biol 3, 512-6.

3.  Ostman, A. and Heldin, C.H. (2001) Adv Cancer Res 80, 1-38.

4.  Betsholtz, C. et al. (2001) Bioessays 23, 494-507.

5.  Coughlin, S.R. et al. (1988) Prog Clin Biol Res 266, 39-45.

6.  Ramalingam, K. et al. (1995) Bioorg Med Chem 3, 1263-72.

7.  Panayotou, G. et al. (1992) EMBO J. 11, 4261-4272.

8.  Kashishian, A. et al. (1992) EMBO J. 11, 1373-1382.


Entrez-Gene Id 5156
Swiss-Prot Acc. P16234


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PathScan® is a trademark of Cell Signaling Technology, Inc.