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Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

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REACTIVITY
Product Includes Volume Cap Color
Bad Capture Ab (100X) 0.4 ml Pink
Bad Detection Ab (100X) 0.4 ml Blue
Anti-mouse IgG, HRP-linked Antibody 0.04 ml Yellow

Product Description

CST's PathScan® Total Bad Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total Bad Sandwich ELISA Kit #7162. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The Bad Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a Bad Detection Antibody and an anti-Mouse IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of total Bad protein.

*Antibodies in this kit are custom formulations specific to the kit.


Product Usage Information

Storage: Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).


1.  Yang, E. et al. (1995) Cell 80, 285-291.

2.  Zha, J. et al. (1996) Cell 87, 619-628.

3.  Datta, S.R. et al. (1997) Cell 91, 231-241.

4.  Peso, L. et al. (1997) Science 278, 687-689.

5.  Bonni, A. et al. (1999) Science 286, 1358-1362.

6.  Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867.

7.  Harada, H. et al. (1999) Mol. Cell 3, 413-422.

8.  Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869.

9.  Lizcano, J. et al. (2000) Biochem. J. 349, 547-557.

10.  Datta, S. et al. (2000) Mol. Cell 6, 41-51.


Entrez-Gene Id 572
Swiss-Prot Acc. Q92934


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PathScan® is a trademark of Cell Signaling Technology, Inc.