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Important Ordering Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

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REACTIVITY
Product Includes Volume Cap Color
IRS-2 Capture Ab (100X) 0.4 ml Pink
IRS-2 Detection Ab (100X) 0.4 ml Blue
HRP-Linked Streptavidin (1000X) 0.04 ml Natural

Product Description

CST's PathScan® Total IRS-2 Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total IRS-2 Sandwich ELISA Kit #7884. Capture and detection antibodies (100X stocks) and HRP-conjugated streptavidin (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IRS-2 rabbit capture antibody is coated in PBS overnight onto a 96 well microplate. After blocking, cell lysate is added followed by an IRS-2 biotinylated rabbit detection antibody and HRP-linked streptavidin. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-2.


Product Usage Information

Storage: Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).


1.  Sun, X.J. et al. (1991) Nature 352, 73-77.

2.  White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.

3.  Withers, D.J. et al. (1998) Nature 391, 900-904.


Entrez-Gene Id 8660
Swiss-Prot Acc. Q9Y4H2


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.

7885
PathScan® Total IRS-2 Sandwich ELISA Antibody Pair