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Important Ordering Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at sales@cellsignal.com.

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REACTIVITY
Product Includes Volume Cap Color
Syk Capture Ab (100X) 0.4 ml Pink
Biotinylated P-Tyrosine Detection Ab (100X) 0.4 ml Blue
HRP-Linked Streptavidin (1000X) 0.04 ml Natural

Product Description

CST's PathScan® Phospho-Syk (panTyr) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-Syk (panTyr) Sandwich ELISA Kit #7928. Capture and detection antibodies (100X stocks) and HRP-linked streptavidin (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The Syk mouse capture antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by biotinylated phospho-tyrosine mouse detection antibody and HRP-linked streptavidin. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of Syk phosphorylated on tyrosines.


Product Usage Information

Storage: Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.


Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl (6,7). Tyr352 of Syk is involved in the association of PLCγ1 (8). Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525/526 of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function (9).


1.  Cheng, A.M. and Chan, A.C. (1997) Curr Opin Immunol 9, 528-33.

2.  Kurosaki, T. (1997) Curr Opin Immunol 9, 309-18.

3.  Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.

4.  Turner, M. et al. (2000) Immunol Today 21, 148-54.

5.  Coopman, P.J. et al. (2000) Nature 406, 742-7.

6.  Deckert, M. et al. (1998) J Biol Chem 273, 8867-74.

7.  Rao, N. et al. (2001) EMBO J 20, 7085-95.

8.  Law, C.L. et al. (1996) Mol Cell Biol 16, 1305-15.

9.  Zhang, J. et al. (2000) J Biol Chem 275, 35442-7.



For Research Use Only. Not For Use In Diagnostic Procedures.
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PathScan® is a trademark of Cell Signaling Technology, Inc.