Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Important Ordering Details

Custom Ordering Details: This product is assembled upon order. Please allow up to three weeks for your product to be processed

Pricing & Additional Information

To learn more about our Blocking Peptides, including pricing, please answer a few questions.

Pricing & Additional Information  

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue using β-Catenin (6B3) Rabbit mAb #9582 in the presence of control peptide (left) or β-Catenin Blocking Peptide (right).

Learn more about how we get our images
Image

Product Description

This peptide is used to block β-Catenin (6B3) Rabbit mAb #9582 and β-Catenin Antibody (Carboxy-terminal Antigen) #9587 reactivity in immunohistochemistry protocols.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks β-Catenin (6B3) Rabbit mAb #9582 and β-Catenin Antibody (Carboxy-terminal Antigen) #9587 by immunohistochemistry.

Product Usage Information

For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).


1.  Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.

2.  Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.

3.  Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.

4.  Amit, S. et al. (2002) Genes Dev 16, 1066-76.

5.  Liu, C. et al. (2002) Cell 108, 837-47.

6.  Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.

7.  Yost, C. et al. (1996) Genes Dev 10, 1443-54.

8.  Morin, P.J. et al. (1997) Science 275, 1787-90.


Entrez-Gene Id 1499
Swiss-Prot Acc. P35222


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.