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Immunohistochemical analysis of paraffin-embedded mouse brain using Neurofilament-L (C28E10) Rabbit mAb #2837 in the presence of control peptide (left) or Neurofilament-L Blocking Peptide (right).

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Product Description

This peptide is used to block Neurofilament-L (C28E10) Rabbit mAb #2837 reactivity in immunohistochemistry protocols.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Neurofilament-L (C28E10) Rabbit mAb #2837 by immunohistochemistry.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols. For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).


1.  Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339.

2.  Hisanaga, S. et al. (1994) Mol. Biol. Cell 5, 161-172.

3.  Sihag, R.K. et al. (1999) J. Neurochem. 72, 491-499.

4.  Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-55.


Entrez-Gene Id 4747
Swiss-Prot Acc. P07196


For Research Use Only. Not For Use In Diagnostic Procedures.
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