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1021S 100 µg

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Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-NF-kappaB p105 (Ser933) (178F3) Rabbit mAb (IHC Specific) #4808 in the presence of control peptide (left) or Phospho-NF-kappaB p105 (Ser933) Blocking Peptide (right).

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Product Description

This peptide is used to block Phospho-NF-kappaB p105 (Ser933) (178F3) Rabbit mAb (IHC Specific) #4808 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-NF-kappaB p105 (Ser933) (178F3) Rabbit mAb (IHC Specific) #4808 by immunohistochemistry.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols. For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).


1.  Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.

2.  Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.

3.  Haskill, S. et al. (1991) Cell 65, 1281-9.

4.  Thompson, J.E. et al. (1995) Cell 80, 573-82.

5.  Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.

6.  Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.

7.  Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.

8.  Chen, Z.J. et al. (1996) Cell 84, 853-62.

9.  Senftleben, U. et al. (2001) Science 293, 1495-9.

10.  Coope, H.J. et al. (2002) EMBO J 21, 5375-85.

11.  Xiao, G. et al. (2001) Mol Cell 7, 401-9.


Entrez-Gene Id 4790
Swiss-Prot Acc. P19838


For Research Use Only. Not For Use In Diagnostic Procedures.
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