Upstream / Downstream

Explore pathways related to this product.

Important Ordering Details

Custom Ordering Details: This product is assembled upon order. Please allow up to three weeks for your product to be processed

Pricing & Additional Information

To learn more about our Blocking Peptides, including pricing, please answer a few questions.

Pricing & Additional Information  

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Immunohistochemical analysis of paraffin-embedded colon carcinoma, using COX IV Antibody #4844 in the presence of control peptide (left) or COX IV Blocking Peptide (right).

Learn more about how we got this image
Image

Product Description

This peptide is used specifically to block COX IV Antibody #4844 and COX IV (3E11) Rabbit mAb #4850 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks COX IV Antibody #4844 and COX IV (3E11) Rabbit mAb #4850 by immunohistochemistry.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols. For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.


1.  Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.

2.  Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.

3.  Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.

4.  Barrientos, A. et al. (2002) Gene 286, 53-63.


Entrez-Gene Id 1327
Swiss-Prot Acc. P13073


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.