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Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 in the presence of control peptide (left) or Phospho-Stat1 (Tyr701) Blocking Peptide (right).

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Product Description

This peptide is used to block Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 signal in immunohistochemistry.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols. For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.


1.  Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.

2.  Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222-227.

3.  Durbin, J.E. et al. (1996) Cell 84, 443-50.

4.  Meraz, M.A. et al. (1996) Cell 84, 431-42.

5.  Wen, Z. et al. (1995) Cell 82, 241-50.

6.  Frank, D.A. (1999) Mol. Med. 5, 432-456.


Entrez-Gene Id 6772
Swiss-Prot Acc. P42224


For Research Use Only. Not For Use In Diagnostic Procedures.
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