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Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue using Thioredoxin 1 (C63C6) Rabbit mAb #2429 in the presence of control peptide (left) or Thioredoxin 1 Blocking Peptide (right).

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Product Description

This peptide is used to block Thioredoxin 1 (C63C6) Rabbit mAb #2429 reactivity in immunohistochemistry protocols.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Thioredoxin 1 (C63C6) Rabbit mAb #2429 signal in immunohistochemistry.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols. For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond that is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (TRX1) and mitochondrial thioredoxin 2 (TRX2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1 (3). Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5).


1.  Watson, W.H. et al. (2004) Toxicol Sci 78, 3-14.

2.  Burke-Gaffney, A. et al. (2005) Trends Pharmacol Sci 26, 398-404.

3.  Saitoh, M. et al. (1998) EMBO J 17, 2596-606.

4.  Callister, M.E. et al. (2007) Intensive Care Med 33, 364-7.

5.  Callister, M.E. et al. (2006) Thorax 61, 521-7.


Entrez-Gene Id 7295
Swiss-Prot Acc. P10599


For Research Use Only. Not For Use In Diagnostic Procedures.
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