Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Important Ordering Details

Custom Ordering Details: This product is assembled upon order. Please allow up to three weeks for your product to be processed

Pricing & Additional Information

To learn more about our Blocking Peptides, including pricing, please answer a few questions.

Pricing & Additional Information  

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Western blot analysis of extracts from serum starved HeLa cells, untreated or IFN-alpha-treated (100 ng/ml for 5 mintues), using Stat1 (42H3) Rabbit mAb #9175 preincubated with an unrelated control peptide (left) or Stat1 Blocking Peptide (#9175 specific) #1079 (right).

Learn more about how we get our images

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Stat1 (42H3) Rabbit mAb (Human Specific) #9175 in the presence of control peptide (left) or Stat1 Blocking Peptide (9175 Specific) (right).

Learn more about how we get our images
Image

Product Description

This peptide is used to block Stat1 (42H3) Rabbit mAb #9175 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Stat1 (42H3) Rabbit mAb #9175 in immunohistochemistry and Western blot.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry and Western blot protocols. For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.

For Western immunoblotting, add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at room

temperature for 30 minutes before allowing to react with the blot.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.


1.  Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.

2.  Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222-227.

3.  Durbin, J.E. et al. (1996) Cell 84, 443-50.

4.  Meraz, M.A. et al. (1996) Cell 84, 431-42.

5.  Wen, Z. et al. (1995) Cell 82, 241-50.

6.  Frank, D.A. (1999) Mol. Med. 5, 432-456.


Entrez-Gene Id 6772
Swiss-Prot Acc. P42224


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.