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Immunohistochemical analysis of paraffin-embedded human lymph node, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 in the presence of control peptide (left) or Phospho-cdc25C (Ser216) Blocking Peptide (right).

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Product Description

This peptide is used to block Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 signal in immunohistochemistry.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols. For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).


1.  Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.

2.  Peng, C.Y. et al. (1997) Science 277, 1501-1505.

3.  Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.

4.  Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.

5.  Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.


Entrez-Gene Id 995
Swiss-Prot Acc. P30307


For Research Use Only. Not For Use In Diagnostic Procedures.
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