Upstream / Downstream

Explore pathways related to this product.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Thanksgiving. We will reopen on Monday, December 1st.

Thank you for your patience.

To Purchase # 1250S

1250S 100 µg $79.00
$ 0. 00

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Western blot analysis of extracts from COS-7 and HeLa cells using PTEN (138G6) Rabbit mAb # 9559 or the same antibody preincubated with PTEN Blocking Peptide.

Learn more about how we get our images

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PTEN (138G8) Rabbit mAb #9559 preincubated with a control peptide (left) or with PTEN Blocking Peptide (right).

Learn more about how we get our images
Image

Product Description

This peptide is used to block PTEN (138G8) Rabbit mAb # 9559 reactivity.


Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks PTEN (138G8) Rabbit mAb #9559 signal in immunohistochemistry.

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols. For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. For western immunoblotting, add 10 µl of antibody and 10 µl of blocking peptide to 10 ml of antibody dilution buffer, and incubate at room temperature for 30 minutes before allowing to react with the blot. Recommended antibody dilutions can be found on the PTEN (138G6) Rabbit mAb #9559 data sheet.


Storage: Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).


Applications: Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols and for western immunoblot analysis.

Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks PTEN (138G8) Rabbit mAb # 9559 signal in immunohistochemistry.

Directions for Use: For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the PTEN (138G6) Rabbit mAb #9559 data sheet.


1.  Wan X and Helman LJ (2003) Oncogene 22, 8205–11

2.  Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.

3.  Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.

4.  Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.

5.  Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.

6.  Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.

7.  Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.

8.  Funamoto, S. et al. (2002) Cell 109, 611-23.

9.  Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.

10.  Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.


Entrez-Gene Id 5728
Swiss-Prot Acc. P60484


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.