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7720S 1050 µl
7720L 5250 µl

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Product Description

Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands between 7 and 175 kDa when electrophoresed (1). The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins. The apparent molecular weight of the prestained proteins are given in the table below.


Product Usage Information

(a) Transfer the desired amount of the Prestained Protein Marker to a separate tube. For visualizing during electrophoresis: use 15 µl for mini-gels and 30 µl for full length gels.

(b) Heat the Marker to 95-100ºC for 3-5 minutes.

(c) After a quick microcentrifuge spin, load directly onto a gel. To ensure uniform mobility, load an equal volume of 1X reducing SDS Loading Buffer into any unused wells.

Note on Apparent Molecular Weights:

The relative sizes of these protein markers may depend on the type of gel used and may appear different than expected. The coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and will likely alter its mobility in an SDS polyacrylamide gel. The extent of this effect can vary with the properties of the gel type (e.g., Tris-glycine, Tris-Tricine, etc) used in the analysis. For this reason, the sizes of these marker proteins are expressed here as apparent molecular weights. For best results, we recommend using these prestained protein markers on a Tris-glycine SDS gel.


Solutions and Reagents:

Contains 0.1–0.2 mg/ml of each protein


Storage: Store at -20°C.

Prestained Protein Marker, Broad Range

Protein Source Apparent MW (Da)
MBP-β-galactosidase E. coli 175,000
MBP-paramyosin E. coli 80,000
MBP-CBD E. coli 58,000
CBD-Mxe Intein-2CBD E. coli 46,000
CBD-Mxe Intein E. coli 30,000
CBD-BmFKBP13 E. coli 25,000
Lysozyme chicken egg white 17,000
Aprotinin bovine lung 7,000
CBD
chitin binding domain
MBP
maltose-binding protein.


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This product is produced at New England Biolabs.