News from the Bench
Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.
Important Ordering DetailsCustom Ordering Details: Sections are cut freshly upon ordering. Please allow up to three business days for your product to be processed.
Our U.S. Offices Are Closed
Our U.S. offices are closed in observance of Thanksgiving. We will reopen on Monday, December 1st.
Thank you for your patience.
To Purchase # 8106S
|8106S||1 Pack (5 slides)||$129.00|
|$ 0. 00|
Find answers on our FAQs page.
PTM information and tools available.
SignalSlide® PTEN IHC Controls #8106
Western blot analysis of extracts from LNCaP or NIH/3T3 cells using PTEN (138G6) Rabbit mAb #9559. NIH/3T3 cells express PTEN and LNCaP cells do not express PTEN. This assay serves to verify PTEN expression.Learn more about how we get our images
Immunohistochemical analysis of paraffin embedded LNCaP (left) and NIH/3T3 (right) cell pellets using PTEN (138G6) Rabbit mAb #9559.Learn more about how we get our images
Gallery: SignalSlide® PTEN IHC Controls #8106
- Western blot analysis of extracts from LNCaP or NIH/3T3 cells using PTEN (138G6) Rabbit mAb #9559. NIH/3T3 cells express PTEN and LNCaP cells do not express PTEN. This assay serves to verify PTEN expression.
- Immunohistochemical analysis of paraffin embedded LNCaP (left) and NIH/3T3 (right) cell pellets using PTEN (138G6) Rabbit mAb #9559.
Each control slide contains formalin fixed, paraffin-embedded LNCaP and NIH/3T3 cell pellets. NIH/3T3 cells express PTEN while LNCaP cells do not express PTEN. Western blot analysis was performed on extracts derived from the same cells to verify PTEN expression.
To be used with antibodies: 9188, 9559.
PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc. SignalSlide® is a trademark of Cell Signaling Technology, Inc.