Upstream / Downstream

Explore pathways related to this product.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Thanksgiving. We will reopen on Monday, December 1st.

Thank you for your patience.

To Purchase # 9183S

9183S 150 µl (10 western blots) $109.00
$ 0. 00

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

MW (kDa)
40 kDa nonphosphorylated, 50 kDa phosphorylated

Western blot analysis of Elk-1 fusion protein expressed from E. coli with or without phosphorylation by purified Erk2 enzyme, using Phospho-Elk-1 (Ser383) Antibody #9186 (upper) or control Elk-1 Antibody #9182 (lower).

Learn more about how we get our images
Image

Product Description

Nonphosphorylated Elk1 fusion protein: Bacterially expressed Elk1 fusion protein serves as a negative control. Supplied in SDS Sample Buffer. Store at -20ºC.

Phosphorylated Elk1 fusion protein: Elk1 phosphorylated by the Erk2 enzyme serves as a positive control. Supplied in SDS Sample Buffer. Store at -20ºC.


Product Usage Information

As controls, we recommend using 15 µl of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.


Storage: Store at -20°C.

The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).


Western Blots: For controls, we recommend using 15 µl of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.


1.  Hill, C.S. and Treisman, R. (1995) Cell 80, 199-211.

2.  Cavigelli, M. et al. (1995) EMBO J. 14, 5957-5964.

3.  Whitmarsh, A.J. et al. (1995) Science 269, 403-407.

4.  Marais, R. et al. (1993) Cell 73, 381-93.

5.  Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.


Entrez-Gene Id 2002
Swiss-Prot Acc. P19419


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.