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|9183S||150 µl (10 western blots)|
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Elk-1 Control Proteins #9183
Gallery: Elk-1 Control Proteins #9183
Nonphosphorylated Elk1 fusion protein: Bacterially expressed Elk1 fusion protein serves as a negative control. Supplied in SDS Sample Buffer. Store at -20ºC.
Phosphorylated Elk1 fusion protein: Elk1 phosphorylated by the Erk2 enzyme serves as a positive control. Supplied in SDS Sample Buffer. Store at -20ºC.
As controls, we recommend using 15 µl of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.Storage: Store at -20°C.
The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).
Western Blots: For controls, we recommend using 15 µl of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.
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