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Western blot analysis of extracts from SK-N-MC cells, untreated (-) or treated with Forskolin #3828 (30 μM) and IBMX (0.5 mM) for 30 min (+), using Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 (upper) and CREB (48H2) Rabbit mAb #9197 (lower).

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Product Description

Nonphosphorylated CREB Cell Extracts: Total cell extracts from SK-N-MC cells prepared without treatment serve as a negative control. Supplied in SDS sample buffer.

Phosphorylated CREB Cell Extracts: Total cell extracts from SK-N-MC cells prepared with Forskolin #3828 and IBMX treatment serve as a positive control. Supplied in SDS sample buffer.


CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).


1.  Lonze, B.E. et al. (2002) Neuron 34, 371-85.

2.  Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.

3.  Redmond, L. et al. (2002) Neuron 34, 999-1010.

4.  Dash, P.K. et al. (1990) Nature 345, 718-21.

5.  Yin, J.C. et al. (1994) Cell 79, 49-58.

6.  Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.

7.  Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.

8.  Ribar, T.J. et al. (2000) J Neurosci 20, RC107.

9.  Tan, Y. et al. (1996) EMBO J 15, 4629-42.



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