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|9293S||100 µl (10 western blots)|
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Bad Control Proteins #9293
Gallery: Bad Control Proteins #9293
Nonphosphorylated Bad Control Proteins: Nonphosphorylated Bad peptide fusion serves as a negative control. Supplied in SDS Sample Buffer.
Phosphorylated Bad Control Proteins: Phosphorylated Bad peptide fusion serves as a positive control. Supplied in SDS Sample Buffer.
As controls, we recommend using 10 µl of phosphorylated and nonphosphorylated Bad control proteins. Boil for 2 minutes prior to use.Storage: Supplied in SDS Sample Buffer:62.5 mM Tris-HCl (pH 6.8 at 25°C),2% w/v SDS, 10% glycerol, 50 mM DTT,0.01% w/v bromophenol blue or phenolred. Store at –20°C.
Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).
Protein Specific References
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.