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MW (kDa)
35

Product Description

c-Jun Fusion Protein Beads selectively "pull down" SAPK/JNK and also serve as a useful substrate to measure SAPK/JNK activity. It is expressed as a recombinant protein fusion to amino acid residues corresponding to c-Jun codons 1-89. The fusion protein is produced in E. coli and is supplied as a 50% slurry of c-Jun Fusion Protein beads.


Product Usage Information

Prior to use, put the tube on ice for 5 minutes to lower viscosity of buffer. Then beads should be resuspended to a 50% slurry by inversion or gentle vortexing.


Solutions and Reagents:

Storage Buffer:

20 mM Tris-HCl (pH 7.4)

150 mM NaCl

1 mM Na2EDTA

1 mM EGTA

1% Triton X-100

2.5 mM sodium pyrophosphate

1 mM β-glycerophosphate

1 mM Na3VO4

1 µg/ml leupeptin

50% glycerol

Note: Please aliquot prior to first use.


Storage: Store at -20°C.

c-Jun is a member of the Jun family containing c-Jun, JunB, and JunD, and is a component of the transcription factor activator protein-1 (AP-1). AP-1 is composed of dimers of Fos, Jun, and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines, and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5), and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival, as well as hypoxia and angiogenesis (8,10). Research studies have implicated c-Jun as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, and rheumatoid arthritis (11,12).


1.  Jochum, W. et al. (2001) Oncogene 20, 2401-12.

2.  Davis, R.J. (2000) Cell 103, 239-52.

3.  Hilberg, F. et al. (1993) Nature 365, 179-81.

4.  Raivich, G. et al. (2004) Neuron 43, 57-67.

5.  Behrens, A. et al. (2002) EMBO J 21, 1782-90.

6.  Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.

7.  Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.

8.  Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.

9.  Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.

10.  Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.

11.  Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.

12.  Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.


Entrez-Gene Id 3725
Swiss-Prot Acc. P05412

Data Sheets & Documentation


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.