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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-IGF-I Receptor β (Tyr1135) (DA7A8) Rabbit mAb 3918 100 µl
H M R 95 Rabbit IgG
IGF-I Receptor β (D23H3) XP® Rabbit mAb 9750 100 µl
H M R Mk 95 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.


Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).


1.  Adams, T.E. et al. (2000) Cell. Mol. Life Sci. 57, 1050-1093.

2.  Baserga, R. et al. (2000) Oncogene 19, 5574-5581.

3.  Scheidegger, K.J. et al. (2000) J. Biol. Chem. 275, 38921-38928.

4.  Hernandez-Sanchez, C. et al. (1995) J. Biol. Chem. 270, 29176-29181.

5.  Lopaczynski, W. et al. (2000) Biochem. Biophys. Res. Commun. 279, 955-960.

6.  Baserga, R. et al. (1999) Exp. Cell Res. 253, 1-6.

7.  White, M.F. et al. (1985) J. Biol. Chem. 260, 9470-9478.

8.  White, M.F. et al. (1988) J. Biol. Chem. 263, 2969-2980.


Entrez-Gene Id 3480, 3643
Swiss-Prot Acc. P08069, P06213


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.