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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb 4991 40 µl
Western Blotting Immunohistochemistry
H M 230 Rabbit IgG
Phospho-VEGF Receptor 2 (Tyr996) Antibody 2474 40 µl
Western Blotting
H M 230 Rabbit 
Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb 3817 40 µl
Western Blotting
H M 230 Rabbit IgG
Phospho-VEGF Receptor 2 (Tyr1175) (D5B11) Rabbit mAb 3770 40 µl
Western Blotting Immunoprecipitation
H M 230 Rabbit IgG
VEGF Receptor 2 (D5B1) Rabbit mAb 9698 40 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R 210, 230 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The Phospho-VEGF Receptor 2 Antibody Sampler Kit provides an economical means of evaluating the VEGFR2 tyrosine kinase and several phosphorylation sites that are involved in its activation. The kit includes enough antibody to perform four western blot experiments with each primary antibody.


Specificity / Sensitivity

The phospho-VEGF Receptor 2 (Tyr1175) (D5B11) Rabbit mAb detects endogenous VEGF Receptor 2 at tyrosine 1175 and may cross-react with other tyrosine-phosphorylated receptor tyrosine kinases, including VEGFR1 and FLT3. The phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb

detects endogenous levels of VEGF Receptor 2 at tyrosine 1059 and can detect VEGF receptors 1 and 3 when phosphorylated at corresponding tyrosine residues due to identical sequences within the epitope region. The phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb detects endogenous VEGF Receptor 2 at tyrosine 951 and may slightly cross-react with activated VEGF receptor 1, but not with other related tyrosine phosphorylated tyrosine kinases. The phospho-VEGF Receptor 2 (Tyr996) Antibody detects transfected VEGF Receptor 2 only when phosphorylated at tyrosine 996. The antibody cross-reacts with tyrosine-phosphorylated PDGF receptor. The VEGF Receptor 2 (D5B1) Rabbit mAb detects endogenous total VEGF Receptor 2.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr996 of human VEGFR2. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr951, Tyr1059 or Tyr1175. VEGFR2 monoclonal antibody is produced by immunizing animals with a recombinant protein containing the carboxy-terminal 150 residues of human VEGFR2.

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).


1.  Meyer, M. et al. (1999) EMBO J 18, 363-74.

2.  Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.

3.  Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.

4.  Takahashi, T. et al. (2001) EMBO J 20, 2768-78.

5.  Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.

6.  Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.

7.  Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.

8.  Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.


Entrez-Gene Id 2321, 3791, 2324
Swiss-Prot Acc. P17948, P35968, P35916


For Research Use Only. Not For Use In Diagnostic Procedures.
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U.S. Patent No. 5,675,063.