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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb 13820 40 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H M R 60 Rabbit IgG
Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb 9516 40 µl
Western Blotting Immunofluorescence Flow Cytometry
H M R 60 Rabbit IgG
Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb 5753 40 µl
Western Blotting Immunoprecipitation
H 60 Rabbit IgG
Smad1 (D59D7) XP® Rabbit mAb 6944 40 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry Chromatin Immunoprecipitation
H M Mk 60 Rabbit IgG
Smad5 (D4G2) Rabbit mAb 12534 40 µl
Western Blotting Immunoprecipitation Chromatin Immunoprecipitation
H M R Mk 60 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

The Smad1/5/9 Antibody Sampler Kit provides an economical means of detecting target proteins of the BMP signaling pathway. The kit contains enough primary antibodies to perform four western blots with each.

Specificity / Sensitivity

Each antibody in the Smad1/5/9 Antibody Sampler Kit recognizes endogenous levels of its respective target. Activation state antibodies detect their intended targets only when phosphorylated at the indicated site(s).

Source / Purification

Phospho-specific monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser206 of human Smad1 protein or Ser463/465 of human Smad1 and Smad5 proteins. Total Smad1, Smad4, and Smad5 monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ser190 of human Smad1, Asp165 of human SMAD4, or Pro249 of human Smad5 protein.

Transforming growth factor-β (TGF-β) superfamily signaling plays a critical role in the regulation of cell growth, differentiation, and development in a wide range of biological systems. In general, signaling is initiated with ligand-induced oligomerization of serine/ threonine receptor kinases and phosphorylation of the cytoplasmic signaling molecules Smad2 and Smad3 for the TGF-β/activin pathway, or Smad1/5/9 for the bone morphogenetic protein (BMP) pathway. Carboxy-terminal phosphorylation of Smads by activated receptors results in their partnering with the common signaling transducer Smad4, and translocation to the nucleus. Activated Smads regulate diverse biological effects by partnering with transcription factors resulting in cell-state specific modulation of transcription (1-7) .

1.  Horbelt, D. et al. (2012) Int J Biochem Cell Biol 44, 469-74.

2.  Ikushima, H. and Miyazono, K. (2010) Nat Rev Cancer 10, 415-24.

3.  Kitisin, K. et al. (2007) Sci STKE 2007, cm1.

4.  Schmierer, B. and Hill, C.S. (2007) Nat Rev Mol Cell Biol 8, 970-82.

5.  Whitman, M. (1998) Genes Dev 12, 2445-62.

6.  Sapkota, G. et al. (2007) Mol Cell 25, 441-54.

7.  Alarcón, C. et al. (2009) Cell 139, 757-69.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

Smad 1/5/9 Antibody Sampler Kit