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To Purchase # 12656S

12656S 1 Kit (6 x 40 µl) $439.00
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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb 13820 40 µl
H M R 60 Rabbit IgG
Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb 9516 40 µl
H M R 60 Rabbit IgG
Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb 5753 40 µl
H 60 Rabbit IgG
Smad1 (D59D7) XP® Rabbit mAb 6944 40 µl
H M Mk 60 Rabbit IgG
Smad4 Antibody 9515 40 µl
H M R Mk 70 Rabbit 
Smad5 (D4G2) Rabbit mAb 12534 40 µl
H M R Mk 60 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
All Goat 

Product Description

The Smad1/5/8 Antibody Sampler Kit provides an economical means of detecting target proteins of the BMP signaling pathway. The kit contains enough primary antibodies to perform four western blots with each.


Specificity / Sensitivity

Each antibody in the Smad1/5/8 Antibody Sampler Kit recognizes endogenous levels of its respective target. Activation state antibodies detect their intended targets only when phosphorylated at the indicated site(s). Phospho-Smad1/5 (Ser463/465) (D5B10) and Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAbs are also predicted to detect Smad9 (Smad8) when phosphorylated at Ser465/467.


Source / Purification

Phospho-specific monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser206 of human Smad1 protein or Ser463/465 of human Smad1 and Smad5 proteins. Total Smad1 and Smad5 monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ser190 of human Smad1 or Pro249 of human Smad5 protein. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro278 of human Smad4. Antibodies are purified by protein A and peptide affinity chromatography.

Transforming growth factor-β (TGF-β) superfamily signaling plays a critical role in the regulation of cell growth, differentiation, and development in a wide range of biological systems. In general, signaling is initiated with ligand-induced oligomerization of serine/ threonine receptor kinases and phosphorylation of the cytoplasmic signaling molecules Smad2 and Smad3 for the TGF-β/activin pathway, or Smad1/5/8 for the bone morphogenetic protein (BMP) pathway. Carboxy-terminal phosphorylation of Smads by activated receptors results in their partnering with the common signaling transducer Smad4, and translocation to the nucleus. Activated Smads regulate diverse biological effects by partnering with transcription factors resulting in cell-state specific modulation of transcription (1-7) .


1.  Horbelt, D. et al. (2012) Int J Biochem Cell Biol 44, 469-74.

2.  Ikushima, H. and Miyazono, K. (2010) Nat Rev Cancer 10, 415-24.

3.  Kitisin, K. et al. (2007) Sci STKE 2007, cm1.

4.  Schmierer, B. and Hill, C.S. (2007) Nat Rev Mol Cell Biol 8, 970-82.

5.  Whitman, M. (1998) Genes Dev 12, 2445-62.

6.  Sapkota, G. et al. (2007) Mol Cell 25, 441-54.

7.  Alarcón, C. et al. (2009) Cell 139, 757-69.


Entrez-Gene Id 4086, 4089, 4090, 4093
Swiss-Prot Acc. Q15797, Q13485, Q99717, O15198


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
XP® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.