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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb 3108 40 µl
H M R Mi 60 Rabbit IgG
Smad2 (D43B4) XP® Rabbit mAb 5339 40 µl
H M R Mk 60 Rabbit IgG
Smad2/3 (D7G7) XP® Rabbit mAb 8685 40 µl
H M R Mk 52, 60 Rabbit IgG
Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb 9520 40 µl
H M R 52 Rabbit IgG
Smad3 (C67H9) Rabbit mAb 9523 40 µl
H M R Mk 52 Rabbit IgG
Smad4 Antibody 9515 40 µl
H M R Mk 70 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
All Goat 

Product Description

The Smad2/3 Antibody Sampler Kit provides an economical means of detecting target proteins of the TGF-β signaling pathway. The kit contains enough primary and secondary antibodies to perform four western blots with each antibody.

Specificity / Sensitivity

Each antibody in the Smad2/3 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Activation state antibodies detect their intended targets only when phosphorylated at the indicated site. The total Smad2, Smad3, and Smad4 antibodies detect their respective targets at endogenous levels.

Source / Purification

Phospho-specific monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser465/467 of human Smad2 and Ser423/425 of human Smad3. Total Smad2 and Smad3 monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the amino termini of mouse Smad2 and Smad3 or His198 of human Smad2/3 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Pro278 of human Smad4. Antibodies are purified by protein A and peptide affinity chromatography.

Transforming growth factor-β (TGF-β) superfamily signaling plays a critical role in the regulation of cell growth, differentiation, and development in a wide range of biological systems. In general, signaling is initiated with ligand-induced oligomerization of serine/ threonine receptor kinases and phosphorylation of the cytoplasmic signaling molecules Smad2 and Smad3 for the TGF-β/activin pathway, or Smad1/5/8 for the bone morphogenetic protein (BMP) pathway. Carboxy-terminal phosphorylation of Smad proteins by activated receptors results in their partnering with the common signaling transducer Smad4, and translocation to the nucleus. Activated Smad proteins regulate diverse biological effects by partnering with transcription factors resulting in cell-state specific modulation of transcription (1-4).

1.  Horbelt, D. et al. (2012) Int J Biochem Cell Biol 44, 469-74.

2.  Ikushima, H. and Miyazono, K. (2010) Nat Rev Cancer 10, 415-24.

3.  Kitisin, K. et al. (2007) Sci STKE 2007, cm1.

4.  Schmierer, B. and Hill, C.S. (2007) Nat Rev Mol Cell Biol 8, 970-82.

Entrez-Gene Id 4087, 4088, 4089
Swiss-Prot Acc. Q15796, P84022, Q13485

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
XP® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.