Upstream / Downstream

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
PSD95 (D27E11) XP® Rabbit mAb 3450 40 µl
Western Blotting Immunofluorescence
H M R 95 Rabbit IgG
Homer1 Antibody 8231 40 µl
Western Blotting
H M R 46 Rabbit 
SHANK2 Antibody 12218 40 µl
Western Blotting Immunoprecipitation
M R 165 Rabbit 
Spinophilin Antibody 9061 40 µl
Western Blotting Immunoprecipitation
H M R Mk 130 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
All Goat 

Product Description

The Neuronal Scaffold Proteins Antibody Sampler Kit provides an economical means of evaluating four major scaffolding proteins. The kit includes enough primary antibody to perform four western blot experiments.


Specificity / Sensitivity

PSD95 (D27E11) XP® Rabbit mAb detects endogenous levels of total PSD95 protein. SHANK2 Antibody recognizes endogenous levels of total SHANK2 protein. Homer1 Antibody detects endogenous levels of total Homer1 protein. This antibody may also detect isoform Homer1c. Spinophilin Antibody recognizes endogenous levels of total spinophilin protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val537 of human SHANK2 protein, residues surrounding Glu130 of human Homer1 protein, or residues surrounding Ala369 of human spinophilin protein. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly99 of human PSD95.

Scaffold proteins are composed of protein-interaction domains that tether multiple components of a signaling pathway to form signal transduction complexes. This organization of signaling molecules can help enhance signaling specificity and speed. Scaffold proteins are central components in neuronal synapses, where dynamic trafficking of synaptic proteins occurs. Mutations in scaffold proteins could have significant impact on synaptic structure and function. Postsynaptic density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins and a scaffolding protein involved in the assembly and function of the postsynaptic density complex (1,2). SHANK proteins act as scaffolds at the neuronal post-synaptic density (PSD), where they play a critical role in PSD assembly of excitatory synapses during development (3,4). While recruitment of SHANK proteins to the synapse is independent of their interaction with Homer (5), proper synaptic targeting of SHANK1 is mediated by interactions between its PDZ domain and PSD proteins (6). Homer proteins (1-3) are scaffolds, composed of an EVH protein–binding domain, a coiled-coil domain and a leucine zipper domain. The EVH domain is a protein-protein binding module that binds to the proline-rich motifs of G-protein–coupled receptors (GPCRs), inositol 1,4,5-triphosphate (IP3) receptors (IP3Rs), ryanodine receptors, and TRP channels (7,8). The coiled-coil and the leucine zipper domains cause multimerization of Homers and assemble signaling proteins complexes. Spinophilin is a protein phosphatase 1 regulatory protein that interacts with a large number of proteins, including ion channel components and G-protein-coupled receptors (GPCRs). Spinophilin also interacts with actin filaments; phosphorylation of spinophilin at Ser94 and Ser177 disrupts this interaction (9,10).


1.  Cao, J. et al. (2005) J Cell Biol 168, 117-26.

2.  Sarrouilhe, D. et al. (2006) Biochimie 88, 1099-113.

3.  Chetkovich, D.M. et al. (2002) J Neurosci 22, 6415-25.

4.  Hsieh-Wilson, L.C. et al. (2003) J Biol Chem 278, 1186-94.

5.  Grabrucker, A.M. et al. (2011) Trends Cell Biol 21, 594-603.

6.  Boeckers, T.M. et al. (1999) J Neurosci 19, 6506-18.

7.  Boeckers, T.M. et al. (2005) J Neurochem 92, 519-24.

8.  Sala, C. et al. (2001) Neuron 31, 115-30.

9.  Fagni, L. et al. (2002) Sci STKE 2002, re8.

10.  Yuan, J.P. et al. (2003) Cell 114, 777-89.


Entrez-Gene Id 9456, 1742, 22941, 84687
Swiss-Prot Acc. Q86YM7, P78352, Q9UPX8-4, Q96SB3


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
XP® is a trademark of Cell Signaling Technology, Inc.